Journal: Kidney360

Article Title: Transforming Growth Factor Beta Receptor 3 (TGFBR3)–Associated Membranous Nephropathy

doi: 10.34067/KID.0001492021

Figure Lengend Snippet: IgG and TGFBR3 colocalize within glomeruli of TGFBR3-associated membranous nephropathy. Representative confocal microscopy images show IgG staining in green (FITC), TGFBR3 staining in red (Rhodamine Red X), and overlay images of the red and green channels in yellow. Staining is shown of a representative (A) TGFBR3-associated MN case (predicted by mass spectrometry), (B) an EXT1/EXT2-associated MN case, and (C) a PLA2R-associated MN case.

Article Snippet: IgG subclass staining used direct FITC conjugates for IgG1 (catalog number 115-095-205; Jackson Immunoresearch), IgG2 (catalog number 115-095-207; Jackson Immunoresearch), IgG3 (catalog number F4641; Sigma), and IgG4 (catalog number F9889; Sigma).

Techniques: Confocal Microscopy, Staining, Mass Spectrometry

Journal: Kidney360

Article Title: Transforming Growth Factor Beta Receptor 3 (TGFBR3)–Associated Membranous Nephropathy

doi: 10.34067/KID.0001492021

Figure Lengend Snippet: TGFBR3-associated membranous nephropathy can occur with or without a proliferative lupus nephritis component. (A) Periodic acid–Schiff stain showing a glomerulus with prominent capillary loops. (B) Hematoxylin and eosin stain showing mesangial expansion within a glomerulus. (C) Jones methenamine silver stain highlighting spikes and holes along the glomerular capillary loops. (D) Periodic acid–Schiff stain showing mesangial hypercellularity. (E) Jones methenamine silver stain displaying endocapillary hypercellularity. (F) Periodic acid–Schiff stain showing cellular crescent formation within a glomerulus. (G) IgG immunofluorescence showing global granular mesangial and capillary loop staining within a glomerulus. (H) TGFBR3 immunofluorescence staining showing global granular capillary loop staining. (I) Subepithelial, intramembranous, and mesangial electron-dense deposits seen by electron microscopy. Original magnification, 400×.

Article Snippet: IgG subclass staining used direct FITC conjugates for IgG1 (catalog number 115-095-205; Jackson Immunoresearch), IgG2 (catalog number 115-095-207; Jackson Immunoresearch), IgG3 (catalog number F4641; Sigma), and IgG4 (catalog number F9889; Sigma).

Techniques: Staining, H&E Stain, Silver Staining, Immunofluorescence, Electron Microscopy

Journal: Kidney360

Article Title: Transforming Growth Factor Beta Receptor 3 (TGFBR3)–Associated Membranous Nephropathy

doi: 10.34067/KID.0001492021

Figure Lengend Snippet: IgG subclass staining shows that TGFBR3-associated membranous nephropathy typically does not express IgG4. IgG subclass staining using FITC-conjugated monoclonal antibodies of a representative TGFBR3-associated MN case is shown.

Article Snippet: IgG subclass staining used direct FITC conjugates for IgG1 (catalog number 115-095-205; Jackson Immunoresearch), IgG2 (catalog number 115-095-207; Jackson Immunoresearch), IgG3 (catalog number F4641; Sigma), and IgG4 (catalog number F9889; Sigma).

Techniques: Staining

Journal: Frontiers in Immunology

Article Title: The Effect of a BSA Conjugate of a Synthetic Hexasaccharide Related to the Fragment of Capsular Polysaccharide of Streptococcus pneumoniae Type 14 on the Activation of Innate and Adaptive Immune Responses

doi: 10.3389/fimmu.2016.00248

Figure Lengend Snippet: Antibody production . CBA mice were immunized intraperitoneally with GC (white bars) or GC + AL (black bars) (10 μg of carbohydrate per mouse) at days 0 and 14 and were boosted on day 92 with the same dose of GC + AL. (A) Anti-PS IgG. Serum samples were collected at the indicated time intervals from mice ( n = 6) and tested by ELISA using plates coated with PS. Antibody titers were expressed as the dilution giving twice the OD obtained for mice on the day 0. The data are shown as the mean ± SEM. (B) IgG1. Individual titers of the anti-PS antibodies on day 28 (day 14 after the second immunization) in BALB/c mice vaccinated with a dose of 10 μg in the presence (black points) or absence (white points) of aluminum hydroxide. Each group contained six mice. The data are shown as individual data points and mean ± SD. One-way ANOVA and Tukey posttest, * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: For secondary antibodies to total IgG, horseradish peroxidase-labeled dry diagnostic antibodies to IgG (H + L) of white mice (Medgamal, Russia) and rabbit anti-mouse peroxidase conjugated IgG1 (gamma 1 chain), IgG2a (gamma 2a chain), IgG 2b (gamma 2b chain), and IgG3 (gamma 3 chain) (Rockland Immunochemicals, Inc., Gilbertsville PA, USA) were used.

Techniques: Enzyme-linked Immunosorbent Assay